Figure 3.
Cytofluorometric detection of mPR3-ANCAs on the surface of primed murine neutrophils. Wild-type peritoneal granulocytes were incubated with mPR3-antiserum pool 1 (solid line) or preimmune serum (dashed line). Enhanced cell surface binding of monospecific antiserum pool 1 in comparison to the binding of a preimmune serum was visualized with a Cy3-conjugated F(ab′)2 goat antimouse (Fab) antibody (left). The right panel shows the absence of staining of peritoneal granulocytes that were prepared from an mPR3/mNE-deficient mouse.
