Summary of percent TCR⁺CD4⁺ cells of total viable lymphocytes (as determined by flow cytometry) as a function of time after vector administration. Examined tissues were thymus (A), spleen (B), portal nodes (C), and inguinal nodes (D). Also graphed are percent TCR⁺CD4⁺ cells of total CD4⁺ cells as a function of time after vector administration (E-H). Examined tissues were thymus (E), spleen (F), portal nodes (G), and inguinal nodes (H). Mice had been transduced with AAV-EF1α-GFP (▪) or AAV-EF1α-ova (•). Antibody staining was carried out using PE-Cy5.5–conjugated anti-CD4 and FITC-conjugated KJ1-26. Results are average ± SD (error bars) for n = 4 mice (day 10) or n = 8 mice (days 30 and 60). Results from portal nodes were average from 2 FACS analyses for nodes pooled from 4 animals (only done for day 30 and 60 time points). (I) Percent apoptotic (annexin V⁺ and propidium iodide^–, AV⁺PI^–TCR⁺) cells of total TCR⁺ cells in the spleens of AAV-EF1α-GFP– or AAV-EF1α-ova–transduced DO11.10 mice (1.5 months after vector administration). Error bars represent SD. * indicates P ≤ .05.