Localization of TLT-1 to platelet and MK α-granules. Resting (top row) or thrombin-stimulated (middle row) platelets or in vitro–derived MKs (bottom row) were permeabilized and then stained with anti–TLT-1 (left column, green) and anti-CD62P (middle column, red) followed by a combination of Alexa 488–conjugated antirabbit and Alexa 633–conjugated antigoat antibodies. The right column is an overlay of the left and middle images.