The combination of FDC-specific mAbs 8D6 and 4G10 inhibits FDC-mediated lymphomagenesis. (A-B) Kinetics of tumor formation. L3055 cells (2 × 106 cells) and HK cells (1 × 106 cells) were injected into nude mice in the presence of medium (i), mAb 8D6 (ii), mAb 4G10 (iii), and mAbs 8D6 plus 4G10 (iv) as indicated. A tumor growth chart (A) and tumor-bearing mice images taken 38 days after tumor cell inoculation (B) are shown. (C) A summary of in vivo tumor formation experiments shows tumor size and incidence. Horizontal bars indicate average tumor volume in each group. (D-E) Seven days after the inoculation of L3055 and HK cells with (E) or without (D) mAbs 8D6 and 4G10, cryosections of tissue from the injection site were prepared for immunofluorescent staining. Alexa-Fluor 488–conjugated mAb 3C8 (green) was used to locate HK cells in tumor tissue. Biotin-conjugated CD38 was used to detect L3055 cells followed by PE-conjugated streptavidin (red). Blue fluorescence dye DAPI was used for nuclei counterstaining. Original magnification, ×200.
