Examples of pulmonary vessels from a sickle mouse. To obtain these images, TF (green), CD31 (red), and nuclei (blue) are overlaid to obtain a merged image in which TF-positive endothelial cells are yellow-orange in color. The vein in the left panel is negative for endothelial TF, whereas the ones in middle and right panels are positive. Note that in middle and, particularly, right images, nonendothelial TF staining has increased prominence as well. Original magnification, × 600.