Effects on IL-6 and IL-10 production in Tax transgenic mouse tumors treated with PS-341. A total of 3 × 106 cells from 5 nontransgenic and 5 Tax transgenic mouse spleen or primary tumor tissues were cultured for 16 hours. Supernatants were used in ELISA to measure (A) IL-6 and (B) IL-10 production. Error bars represent SEM of 1 of 3 separate experiments using tissues from 5 Tax transgenic and 5 nontransgenic mice each. The statistical significance of the inhibition was evaluated by a paired t test: P ≤ .2 for untreated compared to sodium salicylate treatments and P ≤ .1 for untreated compared to PS-341 treatments. The statistical significance for IL-10 production is P ≤ .03 for untreated compared to sodium salicylate treatments and P ≤ .03 for untreated compared to PS-341 treatments.
