Release of tertiary granule MMP-9 is similar in WT and Rac2^-/- neutrophils. (A) Supernatants of WT and Rac2^-/- BMNs, either resting or stimulated with CB, CB with 5 μM fMLP, fibronectin (1 hour), or fibronectin (1 hour) with 5 μM fMLP, were separated by gel electrophoresis and analyzed by zymography to detect gelatinase activity, most of which migrated at approximately 105 kDa. (B) Graph showing the average optical density of MMP-9 activity in supernatants of cells stimulated by CB, CB with 5 μM fMLP, fibronectin adhesion (1 hour), or fibronectin (1 hour) followed by 5 μM fMLP. Error bars indicate SEM.