Effect of anti-Mart on adhesion of U937 cells to immobilized CD11b counterreceptors and ligands and to endothelial cells. (A-C) Immobilized CD11b counterreceptors and ligands. (D) Endothelial cells. Cell adhesion to immobilized purified proteins is shown in the absence of antibody (–) or in the presence of Mac-1–blocking mAbs against CD11b and CD18, purified anti-Mart IgG, and control IgG. Cell adhesion is expressed as absorbance at 590 nm. Cell adhesion to HUVEC monolayer (D) was quantified in the presence of sera 1 and 2 and of IgG as control. Anti-CD11b, anti–ICAM-1, and soluble RAGE were run in parallel. All data are mean ± SD (n = 3) of 3 independent experiments.