Determining anti-Mart glycoprotein specificity in MAIGA assay.Granulocytes from donors who underwent Mart genotyping or from U937 cells were incubated with anti-Mart sera derived from 4 donors (donors 1-4) and mAb bear-1 specific for the CD11b subunit. Bound Mart alloantibodies on CD11b were measured by an antigen capture assay (MAIGA) using peroxidase-labeled antihuman IgG and enzyme substrate. Serum from a healthy blood donor with group AB blood served as a control (AB). All data are means ± SDs (n = 3) of 3 independent experiments.