Scanning electron micrographs reveal that HOCl-LDL alone induces formation of microaggregates; combination with ADP results in stable aggregate formation. Washed platelets were subjected to HOCl-LDL (20 μg/mL), ADP (1 μM), and the combination of the 2 agonists with or without the presence of PKC inhibitor for 30 seconds at 37°C as described for Figure 1 and then fixed with glutaraldehyde for subsequent processing and examination by scanning electron microscopy. (A) Control platelets, (B) ADP alone, (C) HOCl-LDL alone, (D) HOCl-LDL + ADP, (E) HOCl-LDL + bisindolylmaleimide (3 μg/mL), (F) HOCl-LDL + ADP + bisindolylmaleimide. The white bar represents 10 μm for panel A and 1 μm for panels B-F.