Figure 7.
Figure 7. Adhesion of neutrophils to Glu-Pg. Neutrophils were isolated from human blood, resuspended in HBSS, and labeled with calcein. FMLP-(100 nM) activated neutrophils were preincubated with anti-αM mAb 44a (40 μg/mL), anti-α5β1 Ab 1950 (1:200 dilution), or tranexamic acid (5 mM) for 20 minutes at 25° C. Cells were then distributed into the wells coated with Glu-Pg (3.0 μg/mL). Fluorescence of adherent cells was measured as described in “Materials and methods.”

Adhesion of neutrophils to Glu-Pg. Neutrophils were isolated from human blood, resuspended in HBSS, and labeled with calcein. FMLP-(100 nM) activated neutrophils were preincubated with anti-αM mAb 44a (40 μg/mL), anti-α5β1 Ab 1950 (1:200 dilution), or tranexamic acid (5 mM) for 20 minutes at 25° C. Cells were then distributed into the wells coated with Glu-Pg (3.0 μg/mL). Fluorescence of adherent cells was measured as described in “Materials and methods.”

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