Compiled plots of patients with and without GVHD. The data obtained on the samples collected over the observation period were compiled for the patients without complications after HSCT (w/o GVHD) and compared with the polypeptide patterns obtained from patients at diagnosis of GVHD (w/GVHD). Comparison of the 2 patterns shows a number of differentially excreted polypeptides, as visualized here by inserts that magnify the particular parts of the pattern were the changes occur (marked by arrows). Shown are 4 discriminatory peptides, marked in each pattern with A to D and arrows indicating the position of each of these molecules in the compiled patterns, for patients without (w/o) or with (w/) GVHD. Panels A and C show polypeptides newly appearing in all patients with GVHD. Panel B shows a more than 10-fold increase in signal intensity observed in all samples of patients with GVHD, while panel D shows a more than 10-fold reduction of the intensity of the 3.44 kDa/44.1 minutes peptide.