IL-6, IL-8, and TNF-α did not inhibit cleavage of ULVWF multimers under static conditions. ULVWF multimers were incubated with normal plasma or plasma pretreated for 30 minutes at room temperature with IL-6, IL-8, or TNF-α in the presence of urea and barium. After a 24-hour incubation, plasma treated with each cytokine cleaved ULVWF substrate to the same extent as untreated plasma. The figure is a representative of 3 independent experiments.