The effects of the Src kinase inhibitor PP2 and the Syk inhibitor piceatannol on the phosphorylation of pp30 (NTAL) and pp38 (LAT). PP2 (A), piceatannol (B), or carrier solutions were added 10 minutes prior to the addition of 100 ng/mL NP-BSA. The reactions were terminated after 5 minutes by the addition of denaturing lysis buffer. pic indicates piceatannol. (C) Effect of inhibitors on NTAL phosphorylation. HuMCs were sensitized and triggered as described in the legend for Figure 1C, and NTAL phosphorylation was determined as described in the legend for Figure 3A. The blots are representative of 2 to 3 similar experiments. Wort indicates wortmannin; l/c, IgG light chain; and pY, phosphotyrosine.