Effect of PTX3 overexpression on FGF2-T-MAE cell morphogenesis. (A) Aggregates of parental FGF2-T-MAE cells (ctrl), PTX3-overexpressing clones B8 and B9, and mock-transfected clone BVV6 were seeded within 3D-fibrin gels. After 24 hours, aggregates were photographed and sprouts quantified by computerized image analysis. Each point is the mean ± SEM of 4 determinations in duplicate. Student t test, **P < .01. (B) Representative images of aggregates of FGF2-T-MAE (i), B8 (ii), and B9 (iii) cells. Original magnification, × 40.