Frequency of SSp-1–specific CD8⁺ T cells. SSp-1–specific CD8⁺ T-cell frequency among DC-induced human CTLs against SSp-1 (A-B) and splenocytes derived from immunized mice and restimulated for 6 days in vitro (C-D). Tetramer-binding CD8⁺ T cells are shown in the upper-right quadrant and are labeled with the percentage of total CD8⁺ T cells. SSp-1–specific CTLs generated from human PBLs were stained with control HLA-A*0201/CAP-1 tetramers (A) and HLA-A*0201/SSp-1 tetramers (B). Results are representative of 3 independent experiments. Splenocytes from Tg mice immunized with SSp-1–pulsed DCs were stained with control HLA-A*0201/CAP-1 tetramers (C) and HLA-A*0201/SSp-1 tetramers (D).