Histone acetylation profile and transcription factor binding in the mGata1 locus. (A) The mGATA1 locus is shown at the top. The Gata1 gene is depicted as a black box, and the position of cis-elements HS 1, IE promoter, and the intron element (HS4/5) are marked by gray arrows. Below, at different points along the GATA1 locus (x-axis, the coordinates are in kilobases), the degree of enrichment of acetylated histone H3 (black rectangles with 1 SD error bars indicated, y-axis) in eosinophils with intact HS 1 (WT) or deleted for HS 1 (ΔneoΔHS) is shown. Acetylation at HS 1, IE promoter, and HS 4/5 is highlighted by gray stripes. Ns indicates no signal. (B) Above, the mGata1 locus is shown as in panel A. Below, the relative degree of binding (black rectangles with 1 SD error bars indicated, y-axis) of transcription factors GATA1 and C/EBPϵ in wild-type eosinophils and GATA1 in the megakaryocytic cell line, L8057, is shown at different positions along the mGata1 locus (x-axis).