Influence of cytokines and glucocorticoid on TRAIL expression in T cells. T cells were incubated with IFN-γ (50 U/mL), IL-13 (50 ng/mL), or corticosterone (500 nM) for various time durations. (A) RNA was extracted from T cells of SLE patient after an 8-hour incubation with IL-13 (50 ng/mL) or IFN-γ (50 U/mL). INF-γ up-regulated TRAIL expression of T cells. Representative data from 4 individual experiments. (B) RNA was extracted from T cells of an SLE patient with neutropenia after incubation with IFN-γ (50 U/mL) or glucocorticoid (500 nM) for various time durations. IFN-γ up-regulated the TRAIL expression level, while glucocorticoid down-regulated the TRAIL expression in T cells. Shown are representative data from 4 individual experiments.