VAP-1 is not involved in stable adhesion between PMNs and HUVECs. (A) The SSAO inhibitors and (B) anti-VAP-1 mAbs do not have any effects on firm binding of PMNs to VAP-1-transfected cells stimulated with 5 U/mL TNF-α (data from the rolling assay protocol). (C-D) Stronger inflammatory stimulus increases the number of adherent cells, but does not make the binding VAP-1-dependent (data from the transmigration assay protocol). HUVECs transfected with VAP-1 or VAP-1Y471F were pretreated with the various compounds as indicated in the figure, and the numbers (mean ± SEM; n = 3-7) of firmly adherent cells were determined. None of the effects was statistically significant. Endoth. indicates endothelial cells; pretreat., pretreated; and vehic, vehicle.