Western blot. After transfer to the membrane the blot was probed with anti-IVAPLA2 (Santa Cruz Biotechnology) at a 1:300 dilution and peroxidase-conjugated rabbit antimouse at the same dilution. Color development used diaminobenzidine tetra hydrochloride (DAB). Lane 1 shows molecular weight markers; lane 2, U937 cell lysate prior to Prosep column application (3 μg); lane 3, eluate from the Prosep column after U937 application (10 μg); lane 4, SDS elution from U937 Prosep column (7 μg); lane 5, SDS elution from red cell Prosep column (12 μg); lane 6, molecular weight markers; lane 7, platelet preparation (10 μg); lane 8, leukocyte preparation (39 μg). The numbers given in parentheses for each lane refers to the total protein applied to the gel.
