Figure 6.
![Figure 6. Loss of alloreactivity after enrichment and expansion of CMV-specific T cells. (A) CD4+ T cells from either the PBMC fraction (d0) or the generated CMV-specific T-cell lines (later than d10) from 3 donors (LSA7, ▦; LSA8, ▪; LSA5, □) were stimulated with irradiated, mature third-party DCs. Proliferative responses are expressed as the median [3H]-thymidine incorporation (cpm) of the stimulated cells - [3H]-thymidine incorporation of the control. (B) CD8+ T cells purified from the PBMC fraction or the generated CMV-specific T-cell lines were plated with irradiated, mature third-party DCs. Numbers of CD8+ T cells were calculated by cell counting.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/103/9/10.1182_blood-2003-09-3056/6/zh80090460760006.jpeg?Expires=1725025805&Signature=K6PQZ3JlHaI~MCZTHchRutFvAmIw0eXkUmHSxx8kRzhrq2h7xcMeScOghEWD4eAl9COxbyrF7XCb8fXjLvWuAoU0Gqckot799mbnMTzMC9YsP~cAV3dzSTYo6773jUrl~Vp2O7M3p5Qn~ZEertsZXsqmtho-XGCUd~dEp~L6hWcY4AIP-FZcaNlcPNpnWp9b4PLqX-X2cnsUHr-29JQ~n2JbhUgazXl9FbUM0uLJT7Liv8Fo9UkX0~9ioL0V~6LQ4t2U-ZM7c~3Ou6WM9ICXAvMFzeoqFCsAOuFGEQj3hv-bRHYvZr5rFrFQ75CEQ1X-tEvAG8iSe1SVPLdpCB1Zkw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Loss of alloreactivity after enrichment and expansion of CMV-specific T cells. (A) CD4+ T cells from either the PBMC fraction (d0) or the generated CMV-specific T-cell lines (later than d10) from 3 donors (LSA7, ▦; LSA8, ▪; LSA5, □) were stimulated with irradiated, mature third-party DCs. Proliferative responses are expressed as the median [3H]-thymidine incorporation (cpm) of the stimulated cells - [3H]-thymidine incorporation of the control. (B) CD8+ T cells purified from the PBMC fraction or the generated CMV-specific T-cell lines were plated with irradiated, mature third-party DCs. Numbers of CD8+ T cells were calculated by cell counting.
