Effect of GW654652 on patient MM cell survival and migration. (A) Effect of GW654652 on patient MM cell migration. Growth factor-deprived patient MM cells were pretreated with 1 μg/mL GW654652 or were left untreated, plated on a fibronectin-coated polycarbonate membrane (8-μm pore size) in a modified Boyden chamber, and exposed for 4 hours to 5 ng/mL VEGF added to the lower chamber. At the end of treatment, cells on the lower part of the membrane were counted using a Coulter counter ZBII. (open square) Control; (closed square) VEGF; (marked square) VEGF plus GW654652. (B) Effect of GW654652 on patient MM cell survival. Patient MM cells were cultured with control media or the indicated concentrations of GW654652. MTT cleavage was measured during the last 4 hours of 48-hour cultures. Values represent the mean ± SD absorbance of triplicate cultures.