Organization of actin cytoskeleton in normal and WASp-/- osteoclasts. Bone marrow osteoclasts were derived on glass coverslips (A) or bone slices (B), fixed with 3% paraformaldehyde, and permeabilized with 0.05% Triton X-100, and the presence of actin filaments and vinculin was detected by immunofluorescence. We quantified the percentage of cells displaying each of the identified arrangements of actin filaments in 100 cells per experiment. The graphs illustrate the mean percentage of cells ± SD from 3 experiments.