Expression of Mac-1 and Gr-1. Mac-1 expression is shown in the left panel, and Gr-1 expression in the right panel. Flow cytometric analysis was performed on G-CSFR-transfected Ba/F3 and Ba/F3GR cells, which overexpressed Gab2 in the presence of IL-3 or G-CSF for 48 hours. In each panel, the fluorescence with isotype control is shown as a black histogram and the specific antibody as a solid gray line along the x-axis (log scale), and the relative number of cells is shown on the y-axis.