CMLs, but not normal CD34 cells, express HNE. (A) Intracytoplasmic HNE in CML-CD34⁺ cells (HLA-identical sibling of donor 7) was identified by flow cytometry. Positive control, HL60 cells; negative controls, LCL and normal G-CSF mobilized PBMC CD34⁺ cells. Filled histogram indicates IgG1-FITC control. Mean fluorescence intensity (MFI) of each sample and IgG1 control is shown in italics and parentheses, respectively. (B) mRNA detection: lanes 1-2, G-CSF-mobilized CD34⁺ cells from 2 healthy donors; lanes 3-4, CML-CD34⁺ cells from 2 patients in chronic phase; lane 5, HL60-positive control; lane 6, dH₂O, GAPDH (glyceraldehyde-3-phosphate dehydrogenase) quantitative control for RNA.