Induction of HNE-specific CTLs in 2 donors. (A-C) Donor 7. (A) After 4 stimulations, CD3⁺ T cells showed dose-dependent cytotoxicity to HNE-loaded autologous monocytes. No response was noted with KLH, unloaded APCs, or K562 cells. Representative data from 3 independent experiments are shown. (B) Cold target inhibition assay to exclude nonspecific killing by NK and NK-T cells confirmed HNE-specific cytotoxicity by CTLs. (C) Abrogation of CTL activity by 10 μg antihuman HLA class I antibody (23.9% versus 3.9%, respectively). Data represent mean ± SD from 3 independent experiments. (D-E) Donor 12 (HLA; A03,-, B07,15, Cw03,0702, DRβ1^*01,^*1001, DQβ1^*05,^*1). HNE-specific CTLs were induced after 4 stimulations. Representative data are from 3 independent experiments.