Contribution of WT SCL- and DN SCL-transduced donor-derived cells in myeloid and lymphoid lineages 6 weeks after transplantation. (A) Results of representative FACS analysis of peripheral blood. Profiles for GFP and Mac1 or Gr1 in peripheral blood cells (upper panels) and B220 and Thy1.2 in GFP⁺-gated peripheral blood subpopulations (lower panels) are shown. (B) Mean ratios of T, B, and myeloid lineage cells in GFP⁺-gated, nongated, or GFP^--gated peripheral blood subpopulations in recipient mice receiving Mock GFP-transduced cells (SD = 0.02-0.04), WT SCL-transduced cells (SD = 0.19-0.60), and DN SCL-transduced-cells (SD = 0.21-0.22). (C) Results of representative FACS analysis of bone marrow and spleen. (D) Chimerism of GFP⁺ cells in myeloid cells from peripheral blood and bone marrow (upper panels), in B220⁺ cells from spleen (lower left), and in Thy1.2⁺ cells from thymus (lower right). (A-D) Three, 7, and 5 recipient mice were analyzed during Mock GFP-transduced, WT SCL-transduced, and DN SCL-transduced cell transplantation, respectively. Numbers in each quadrant (A,C) represent the percentage of cells in the respective quadrants.