Sensitivity of decrypted TF to TFPI and annexin V. (A) HEK293 cells stably expressing a GPI-anchored version of TFPI (TFPI-PI) were treated with cell buffer alone or cell buffer containing 10 mM MBCD or 1% octyl glucoside. Aliquots of the treated cells were analyzed for fVIIa/TF activity in the presence (+) or absence (-) of 1 μg/mL inhibitory monoclonal antibody against the first Kunitz domain of TFPI. Rates of fVIIa/TF activity are expressed as ratios to buffer-treated cells in the absence of inhibitory antibody. Data presented are means ± 1 SDs for 6 determinations. (B) Wild-type HEK293 cells were treated with cell buffer alone (•) or cell buffer in the presence of 10 mM MBCD (○) or 1% octyl glucoside (▾). Aliquots of the treated cell suspensions were analyzed for fVIIa/TF activity in the presence of the indicated concentrations of annexin V. Rates of TF activity are expressed as ratios to buffer-treated cells in the absence of annexin V. Data presented are means ± SEM for at least 4 determinations.