MBCD does not affect cell surface TF content but enhances exposure of phosphatidylserine. Wild-type HEK293 cells were treated (107/mL) with cell buffer alone or with cell buffer containing 10 mM MBCD for 1 hour. In both the left and right panels, histogram a (black) represents buffer-treated cells stained with antimouse IgG-PE. Cells treated with MBCD and stained with antimouse IgG-PE displayed a distribution identical to histogram a. (Left) Histograms b (gray) and c (boldface) show cells stained consecutively with monoclonal anti-TF antibody and antimouse IgG-PE following treatment with buffer or 10 mM MBCD, respectively. (Right) Histogram b (gray) and c (boldface) show cells stained with annexin V–PE following treatment with cell buffer or MBCD, respectively.
