FVIIa-induced IL-8 expression in breast carcinoma cells. Quiescent monolayers of MDA-MB-231 cells were treated with varying concentrations of FVIIa (A-C), a plasma concentration (10 nM) of zymogen FVII or FVIIa (D), or varying concentrations of active site-inactivated FVIIa (ASIS/FFR-FVIIa), followed by 10 nM FVIIa (E). (F) Cells were pretreated with anti-TF IgG or control IgG (100 μg/mL) for 45 minutes before FVIIa (10 nM) was added to the cells. Unless otherwise specified, cells were treated with FVIIa for 75 minutes at 37°C, and total RNA was isolated and subjected to Northern blot analysis. (A, D-F) Representative Northern blot analysis of IL-8 mRNA; (B) quantitative data from such experiments. (C) IL-8 antigen levels in overlying conditioned media of MDA-MB-231 cells treated with varying concentrations of FVIIa. Error bars indicate SEM from 2 to 3 experiments.