Analysis of myeloerythroid progenitors after osteoblast depletion. Bone marrow, spleen, and liver suspensions from Col2.3ΔTK mice treated with PBS (□) or GCV (▦) were plated in methylcellulose media supplemented with CSF, IL-3, IL-6, and erythropoietin (Methocult M3434; StemCell Technologies). After 10 days of culture, the total number of colonies were counted as a representation of early myeloerythroid progenitors. The experiment was done after 25 days of treatment and includes 3 to 5 mice per experimental group.