Figure 7.
![Figure 7. The impact of p110δ inhibition or deletion on PIP3 production in stimulated neutrophils. (A) Thin-layer chromatography of phospholipid extracts from neutrophils purified from WT or p110δ-deficient animals. Cells labeled with [32P] were pretreated with IC87114 (10 μM) or vehicle control prior to activation with fMLP (1 μM). PIP3 standard is shown for comparison. (B) PIP3 quantitation. Results are expressed as a percentage of total phospholipids and represent 2 independent experiments performed in duplicate.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/103/9/10.1182_blood-2003-05-1667/6/zh80090460640007.jpeg?Expires=1723911666&Signature=4hlw9T1x1SdlZW3w2althpTZ3EU3DWE-pn5BEYqVTMy7astXN0o0-IIaGjqGg1VBN-6KhNsQqvDePSg-1oY0Z0ZOe48lB2I4Dg05GLdF6fLfnSlZ-YDuSytNbBgZUbv5KUQfVPDKRgsuFDMeINd3UMV4P4J-Y~VT5pFsLjSBmnHJ0tICiWv7yUwNXqgc66pX4mNENHeSG5k1RYim8L6fEMzGZzvvOxKQX2yM2m8MX9cqk4THDEYcWQPKVosMIMIa7nolkxyImQIcGvI9HAyBZisKrC~EKKnAgi9cVUotPrHcNWcCh4fKRzcVmVECISVQg9qp88EDq~59lsLE-CUgkQ__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
The impact of p110δ inhibition or deletion on PIP3 production in stimulated neutrophils. (A) Thin-layer chromatography of phospholipid extracts from neutrophils purified from WT or p110δ-deficient animals. Cells labeled with [32P] were pretreated with IC87114 (10 μM) or vehicle control prior to activation with fMLP (1 μM). PIP3 standard is shown for comparison. (B) PIP3 quantitation. Results are expressed as a percentage of total phospholipids and represent 2 independent experiments performed in duplicate.
