Cooperation between MT1-MMP and Gαi for the induction of morphogenic differentiation and EC migration. (A) BAECs were cotransfected with either an empty vector (pcDNA3.1) or an MT1-MMP construct, and with either pcDNA3.1, a constitutively active Gαi2 mutant (Gi2-Q205L), or wild-type Gαi2 and allowed to recover for 40 hours. Cotransfected cells were plated on Matrigel in serum-free medium. Original magnification, × 50. (ii) Formation of capillary-like structures was recorded after 6 hours and quantified using a computer-based program. Data are expressed as x-fold induction ± SD of control (pcDNA3.1). Results are the means of 2 independent experiments. (B) Cotransfected cells were allowed to migrate for 3 hours in serum-free medium. Cell migration was quantified. Data are expressed as x-fold induction ± SD of control (pcDNA3.1). Results are the means of 2 independent experiments.
