Figure 3.
![Figure 3. Mutation analysis of β/SMMHC for the interaction with the Runt domain. (A) Identification of the second binding domain in β/SMMHC. GST-tagged Runt domain (GST-RD[25-190]) was mixed with various deletion constructs of β/SMMHC, and GST pull-down experiment was performed. Lanes 1 to 12 indicate 10% of the input of in vitro– translated β/SMMHC or its derivatives; lanes 13 to 24, control pull-down experiment with GST alone; and lanes 25 to 36, GST pull-down experiment using GST-RD(25-190). (B) The schematic diagram of β/SMMHC-derived constructs and the result of the GST pull-down experiments. The binding activities of each construct are listed to the right. X marks in panel B indicate mutations.](https://ash.silverchair-cdn.com/ash/content_public/journal/blood/103/8/10.1182_blood-2003-07-2188/6/zh80080459760003.jpeg?Expires=1723271307&Signature=q56ktMdinF~Bu5~5Hm6KEZtQj1Qp76xq2rUO-azwPcIL~om0UvdKSBduXO0dePXLsXBudIh8i-uSD9tdFQWzbpuczAJq0vdooadvRT8PbCJ6s23TbGB-EZjmZlwv2ZTJFDQylWFhiiJheqPeQAv8exOUPhmYDovM9bhUADwmICNf3SOutC1NuON1tI4387I4yGPFZjlFizlR9vsLIdGnZ3hpxmF95mSBoJvxeSAztFrghbh1FaCJEO1yEFX0SoNWCFDxRk~HGV9Rg4ZQLz99fZao3ZBgVArNc8mzkC52koOhk7YbkHYZ5QCNhfJGKsCSEc9fKi5kYbhLSSZ3KV3sGw__&Key-Pair-Id=APKAIE5G5CRDK6RD3PGA)
Mutation analysis of β/SMMHC for the interaction with the Runt domain. (A) Identification of the second binding domain in β/SMMHC. GST-tagged Runt domain (GST-RD[25-190]) was mixed with various deletion constructs of β/SMMHC, and GST pull-down experiment was performed. Lanes 1 to 12 indicate 10% of the input of in vitro– translated β/SMMHC or its derivatives; lanes 13 to 24, control pull-down experiment with GST alone; and lanes 25 to 36, GST pull-down experiment using GST-RD(25-190). (B) The schematic diagram of β/SMMHC-derived constructs and the result of the GST pull-down experiments. The binding activities of each construct are listed to the right. X marks in panel B indicate mutations.
