The effects of survival factors on p70S6K and Akt phosphorylation in cells pretreated with rapamycin and/or dexamethasone. Karpas 707 cells were pretreated with rapamycin in the absence or presence of dexamethasone for 1 hour, before incubation with rIGF-I, LongR3-IGF-I (LR3), or rIL-6 for 10 minutes followed by lysis. The extracts were analyzed by Western blotting using antibodies specific for the p70S6K phosphorylation sites Thr389 and Thr421/Ser424 and the Akt phosphorylation site Ser473.