The effects of long-term rapamycin treatment on survivin and bcl-xL expression and on apoptosis. (A) Karpas 707 cells were incubated in the presence of rapamycin. Every 48 hours half of the spent medium was exchanged together with addition of fresh rapamycin. At 0, 6, 24, 48, 72, 96, and 120 hours cells were lysed, and extracts were subjected to Western blotting analysis by using antibodies specific for the antiapoptotic proteins survivin and bcl-xL. (B) Karpas 707 and U-1958 cells were treated with rapamycin as described earlier, and cells were harvested at 144 hours. Apoptosis was analyzed by AV/PI staining, and the cells were categorized as described in the legend for Figure 2. The relative cell number is presented as the percentage of 10 000 cells. Three independent experiments per cell line were performed and one representative is shown.
