Figure 6.
Figure 6. Uninfected red cells inhibit early stages of the IFN-γ response to malaria antigens. IFN-γ (pg/mL) was assayed in supernatants of PBMCs cultured for 6 days with 5 × 105/mL iRBCs or 5 × 105/mL PfSL, PPD, or control antigens (not shown). PBMCs were cultured alone or with uninfected red blood cells (equivalent to 1:10 dilution) added to cultures immediately (time 0), after 30 minutes, or after 2, 6, 24, or 72 hours. Data from 5 donors are shown. The dotted line indicates the LLD for the IFN-γ ELISA (125 pg/mL).

Uninfected red cells inhibit early stages of the IFN-γ response to malaria antigens. IFN-γ (pg/mL) was assayed in supernatants of PBMCs cultured for 6 days with 5 × 105/mL iRBCs or 5 × 105/mL PfSL, PPD, or control antigens (not shown). PBMCs were cultured alone or with uninfected red blood cells (equivalent to 1:10 dilution) added to cultures immediately (time 0), after 30 minutes, or after 2, 6, 24, or 72 hours. Data from 5 donors are shown. The dotted line indicates the LLD for the IFN-γ ELISA (125 pg/mL).

Close Modal
This Feature Is Available To Subscribers Only

or Create an Account

Close Modal
Close Modal