IL-3–dependent expression of annexin II.Baf-3 cells (A) or FL5.12 cells (B) were cultured in IL-3–free medium for the indicated times. (Top 2 panels) Northern blot analysis of total RNA (20 μg). The blot was hybridized with an annexin II cDNA probe and then rehybridized with a β-actin probe. (Lower 2 panels) Immunoblot analysis to detect annexin II and α-tubulin proteins.