Correlation between MVD and the tumor load, paraprotein, and BrdU incorporation. (A) Correlation between MVD and tumor load during 5T2MM disease progression. Tumor load was assessed as described in the legend of Figure 1. BM samples from the same mice were stained for CD31, and MVD (number of blood vessels per 0.20 mm2) was quantified. Each point represents mean value of 3 animals. (B) Correlation between MVD and paraprotein during 5T2MM disease progression. MVD was measured as described in “Materials and methods.” Before killing, the animals were bled and serum paraprotein concentration was quantified by protein electrophoresis. Each point represents mean values of 3 mice. (C) Correlation between MVD and BrdU incorporation during 5T2MM disease progression. MVD was quantified as described in panel A. 5T2MM cells from different disease stages were sorted by flow cytometry. Cells were incubated with BrdU for 1 hour followed by flow cytometric analysis of nuclei. Each point represents data from one mouse.
