RT-PCR confirmation of various novel genes enriched in all 6 huES cell lines. (A) Expression of Nanog (FLJ12581) and 3 novel genes (KIAA1573, MGC27165, and GSH1), (B) KIAA1265 and Zf43 genes, and (C) TNNT1, Laminin receptor, ARL8, PPAT, Numatrin, HNRPA1, and TD-60. β-actin served as an internal control and RT without input DNA as a negative control.