American Society of Hematology

Figure 1.

$Figure 1. Cytokine expression in productively infected cells. (A) Enrichment of CD4+ T cells. CD4+ T cells were enriched by positive immunoselection from healthy donors. Left histogram represents isotype-matched controls, and right histogram represents CD4 after selection. (B) Discrimination between p24+ and p24- fractions. Purified CD4+ T cells were either mock infected or infected with HIV and then stimulated with anti-CD3/CD28. At 6 days after infection or mock infection, the cells were stained for intracellular p24. (C) Cytokine expression in the 3 subpopulations. Infected CD4+ T cells were treated as described for panel B and stained for surface CD4 and intracellular IL-2, IL-4, IL-10, or IFN-γ along with HIV p24. Data are from 1 donor, representative of at least 3 donors.$

Cytokine expression in productively infected cells. (A) Enrichment of CD4⁺ T cells. CD4⁺ T cells were enriched by positive immunoselection from healthy donors. Left histogram represents isotype-matched controls, and right histogram represents CD4 after selection. (B) Discrimination between p24⁺ and p24^- fractions. Purified CD4⁺ T cells were either mock infected or infected with HIV and then stimulated with anti-CD3/CD28. At 6 days after infection or mock infection, the cells were stained for intracellular p24. (C) Cytokine expression in the 3 subpopulations. Infected CD4⁺ T cells were treated as described for panel B and stained for surface CD4 and intracellular IL-2, IL-4, IL-10, or IFN-γ along with HIV p24. Data are from 1 donor, representative of at least 3 donors.

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