MSP of cell lines and primary myeloma samples. (A) MSP for SOCS1 with primers MSP5′, showing that methylation was absent in the cell lines and primary myeloma samples tested. B indicates blank; P, positive control; MM, primary multiple myeloma samples. (B) MSP for SHP1, showing that with the exception of HL60 all the cell lines were methylated. All 5 of the myeloma samples (MM1 to MM5) were methylated.