HUVEC migration in the presence of RGDS. FGF-2-induced HUVEC migration across collagen IV (10 μg/mL) was examined in the presence of increasing doses of RGDS. Data are expressed as mean ± SE of 3 experiments carried out in duplicate. (A) RGDS reduced in a dose-dependent manner cell migration (^*P < .05 and ^**P < .01 at 250 μg/mL or 500 μg/mL, respectively). (B) The antichemotactic effect was maintained on HUVECs pretreated with the peptide (500 μg/mL) for 20 minutes at 37°C and then washed with high ionic strength (^*P < .01). (C) HUVEC migration across collagen IV (10 μg/mL) in the presence of RGDS (500 μg/mL) was examined. Migration was induced by FGF-2 (10 ng/mL) and cells were pretreated for 20 minutes at 37°C with caspase inhibitors. A pan-caspase inhibitor (Z-VAD-FMK, 50 μM) treatment abolished the RGDS inhibitory effect (^*P < .01). (D) Specific caspase 8 and 9 inhibitors (named “8” and “9,” respectively) reverted the inhibitory effect of RGDS, whereas caspase 1 and 3 inhibitors (named “1” and “3”) had no effect (^*P < .05).