Analysis of gene expression in CD34⁺CD38^- cells derived from human ESCs and adult BM. Cytoplasmic RNA from CD34⁺ CD38^- cells was used to construct cDNA pools, and the expression of genes was examined by semiquantitative PCR. The number at the top of each lane indicates the amount (microliters) of cDNA used in the 50-μL PCR reaction. M = 1 kb plus DNA ladder.