Immunofluorescence flow cytometric gate profiles for CD34⁺CD38^- cells. (A) Isotype antibody controls for cells derived from ESC differentiation. (B-C) FITC-CD34 and PE-CD38 antibodies for cells derived from ESC differentiation (B) and for cells of human bone marrow (C). Cells in gate R2 are defined as CD34⁺CD38^- cells.