Deletion analysis of the -950 EKLF promoter. The images at the top of the figure show the typical appearances of positive and negative d10.5 and d13.5 embryos. At the bottom of the figure are schematic diagrams of the deletions/point mutations analyzed in the study. The results from analyzing embryos derived from multiple lines (positive/total lines examined) are shown for each construct after analysis at either d10.5 or d13.5. EHS1 and EHS2 are the locations of erythroid-specific DNase hypersensitive sites as described in “Results,” and the GATA site point mutation in the minimal promoter is also shown. Original magnification, × 50 (left); and × 20 (right).