NPM-ALK inhibits 3X IRS promoter activation by FOXO3a. U-2OS cells were transfected with the 3 × IRS reporter plasmid and pCMV-Renilla along with either 0.02, 0.1, or 0.5 μg pcDNA-NPM-ALK in the presence or absence of 0.1 μg pcDNA-FOXO3a. Cell lysates were prepared, and luciferase activity was measured and normalized to Renilla luciferase activity. Results shown are representative of 3 independent experiments, ± SD.