Figure 2.
Figure 2. Rapid dedifferentiation of HEVECs outside the lymphoid tissue microenvironment. Semiquantitative RT-PCR comparison of key HEV genes mRNA levels between HEVECs freshly isolated from human tonsils without any cell culture step (D0 HEVECs) and HEVECs cultured for 2 days (D2 HEVECs). Numbers above the gels indicate PCR cycles used for each gene. Analyzed HEV genes include chemokine receptor DARC (DARC used as a positive control together with GAPDH as standard; panel A), fucosyltransferase FucT-VII (FucT-VII; panel B), L-selectin ligand sulfotransferase LSST/HEC-GlcNAc6ST and Core1 extending β(1-3)–N-acetylglucosaminyltransferase (LSST and Core1-β3GlcNAcT; panel C), and chemokine SLC/CCL-21 (CCL-21; panel D). *Primer multimers that do not correspond to the correct amplification products.

Rapid dedifferentiation of HEVECs outside the lymphoid tissue microenvironment. Semiquantitative RT-PCR comparison of key HEV genes mRNA levels between HEVECs freshly isolated from human tonsils without any cell culture step (D0 HEVECs) and HEVECs cultured for 2 days (D2 HEVECs). Numbers above the gels indicate PCR cycles used for each gene. Analyzed HEV genes include chemokine receptor DARC (DARC used as a positive control together with GAPDH as standard; panel A), fucosyltransferase FucT-VII (FucT-VII; panel B), L-selectin ligand sulfotransferase LSST/HEC-GlcNAc6ST and Core1 extending β(1-3)–N-acetylglucosaminyltransferase (LSST and Core1-β3GlcNAcT; panel C), and chemokine SLC/CCL-21 (CCL-21; panel D). *Primer multimers that do not correspond to the correct amplification products.

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