Enforced expression of TRAF6 enhances LPS-induced activation of Akt in the absence of functional NF-κB. Double-transduced endothelial cells (as labeled) were treated with LPS (100 ng/mL) and CHX (50 μg/mL) for various times as indicated. Activation of Akt (A) and ERK (B) was determined by immunoblotting for phospho-Akt or phospho-ERK, respectively. Membranes were stripped and reprobed for α-tubulin as a loading control. Results are representative of 3 independent experiments.