Tgf-β1^-/- CD4⁺CD25⁺ T cells attenuate inflammation in Rag2^-/- recipients of CD25-depleted Tgf-β1^-/- cells. (A) The percentage body weight (relative to start weight) of Rag2^-/- recipient mice is shown (Tgf-β1^-/- CD25-depleted; •) and indicates suppression of disease transfer by Tgf-β1^-/- (▪) and Tgf-β1^+/+ (▴) CD4⁺CD25⁺ T cells. (B) H&E-stained sections of ears (i-iii) show diminished inflammation and swelling in both groups of Rag2^-/- recipients receiving suppressor cells (ii-iii); immunohistochemical staining for CD3 in the heart (iv-vi) and liver (vii-ix) also show suppression of infiltration of T cells into these organs; immunohistochemical staining of lung sections for CD8 (x-xii) and of CD4 (xiii-xv) reveals tissue infiltrates of each subset and suppression by Tgf-β1^-/- and Tgf-β1^+/+ CD4⁺CD25⁺ T cells. Original magnifications: × 100 (i-iii); × 400 (iv-xv). (C) Serum ALT levels of Rag2^-/- recipients are shown. Horizontal bars indicate average value. (D) Serum albumin levels of Rag2^-/- recipients are shown. Error bars (A, C-D) indicate SD.